Postdoctoral position with Kirsten Skarstad- DNA compaction- Oslo
Postdoctoral position available at Kirsten Skarstad lab in Oslo. If you want to know more write to firstname.lastname@example.org and we will give you the contact information.
Understanding bacterial DNA condensation induced by the SOS response and identifying new targets for development of antibiotics and adjuvants
This project aims to identify proteins and gene regulatory networks involved in DNA compaction through high-content imaging (HCI) of all 4 387 E. coli mutants in the Keio collection. Key proteins will be characterized as possible antibiotic targets.
Antimicrobial resistance (AMR) is increasing worldwide and possesses a threat to global health and economy. Bacteria have developed multiple resistance mechanisms to current antibiotics, showing the need for adjuvants and new antibiotics that target alternative metabolic pathways. One of the mechanisms that allow bacteria to survive and acquire drug resistance is the SOS response. Early stages of this response include an extensive reorganization and compaction of the DNA, possibly to facilitate recombination and repair of damaged DNA. This project aims to identify proteins and gene regulatory networks involved in DNA compaction as potential antibiotic targets through high-content imaging (HCI) of all 4387 E. coli mutants in the Keio collection. These mutants are exposed to sub-lethal doses of the fluoroquinolone ciprofloxacin, which triggers SOS response, and the occurrence of DNA compaction is analyzed. These findings could translate into new potential targets for antibiotics to address the growing threat of AMR.